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Plasmonic Sensing of Biological Analytes Through Nanoholes
February 2008
Grace M. Hwang, The MITRE Corporation
Lin Pang, University of California
Elaine H. Mullen, The MITRE Corporation
Yeshaiahu Fainman, University of California
ABSTRACT
A transmission-based surface plasmon resonance (SPR) sensor for label-free detection of protein-carbohydrate and protein-protein binding proximate to a perforated gold surface is demonstrated. An SPR instrument makes real-time measurements of the resonant wavelength and/or the resonant angle of incidence of transmitted light; both are influenced by the presence of proteins at the gold surface-liquid interface.
Ethylene glycol solutions with known refractive indices were used to calibrate the instrument. A paired polarizationsensitive
detector achieved an overall detection resolution of ~6.6 X 10-5 refractive index units (RIU). Proof of principle experiments were performed with concanavalin A (Con A) binding to gold-adsorbed ovomucoid and anti-bovine serum albumin (BSA) binding to gold-adsorbed BSA.
Publication
Published in 2008. IEEE Sensors Journal, Vol. 8, pp. 2074-2079.
Additional Search Keywords
Surface plasmon resonance (SPR), surface plasmon polariton (SPP), gold nanohole array, bioplasmonics, biosensors
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